CMV pp65 protein (His tag) (80-1366)

Purified recombinant CMV pp65 protein (His tag)

Synonyms UL83 protein, Cytomegalovirus pp65 protein, Cytomegalovirus UL83 protein, CMV Pp65 protein, CMV Pp65 antigen
Species Viral
Protein Type Recombinant
Applications Dot Blot, ELISA, ICC, WB

Specifications

Residues pp65 expressed as a recombinant antigen fused to a his-tag in its N-terminus. Produced from the major exon of UL83 gene
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 51.902 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in 20 mM phosphate buffer, pH 8.0, with 1 M NaCl and 0.1% polyoxyethelene (10) tridecyl ether and 6 mM urea
Concentration 1 - 2 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:1500
Bioactivity Immunoreactive with sera of CMV-infected individuals

Storage & Safety

Storage Store at -20 deg C to -80 deg C, avoid repeated freeze/thaw cycles

General Information

Biological Significance pp65 (UL83) is a phosphoprotein located in the nucleolar matrix of lytically infected fibroblasts. This tegument protein pp65 was found to be most prominent at early times after HCMV infection.

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