CMV pp65 protein (His tag) (80-1366)
Purified recombinant CMV pp65 protein (His tag)
Overview
Overview
| Synonyms | UL83 protein, Cytomegalovirus pp65 protein, Cytomegalovirus UL83 protein, CMV Pp65 protein, CMV Pp65 antigen |
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| Species | Viral |
| Protein Type | Recombinant |
| Applications | Dot Blot, ELISA, ICC, WB |
Specifications
| Residues | pp65 expressed as a recombinant antigen fused to a his-tag in its N-terminus. Produced from the major exon of UL83 gene |
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| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Molecular Weight | 51.902 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied in 20 mM phosphate buffer, pH 8.0, with 1 M NaCl and 0.1% polyoxyethelene (10) tridecyl ether and 6 mM urea |
| Concentration | 1 - 2 mg/ml |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:1500 |
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| Bioactivity | Immunoreactive with sera of CMV-infected individuals |
Storage & Safety
| Storage | Store at -20 deg C to -80 deg C, avoid repeated freeze/thaw cycles |
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General Information
| Biological Significance | pp65 (UL83) is a phosphoprotein located in the nucleolar matrix of lytically infected fibroblasts. This tegument protein pp65 was found to be most prominent at early times after HCMV infection. |
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