cMyc antibody (10-2499)

Mouse monoclonal cMyc antibody

Synonyms Monoclonal cMyc antibody, Anti-cMyc antibody, MYC, MRTL, MYCC, bHLHe39, c-Myc antibody
Specificity Human
Applications ChIP, ELISA, IHC, IP, WB
Immunogen cMyc antibody was raised in Mouse using synthetic peptide (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA - 32 aa) corresponding to 408-439 of Human c-Myc conjugated to KLH as the immunogen

Images

Western Blot analysis using cMyc antibody (10-2499)

Immunoblotting assay of myc mAbs. COLO 320 HSR cells were dissolved in SDS buffer and fractionated on a 12.5% SDS-polyacrylamide gel. Fractionated proteins were electroblotted onto nitrocellulose paper, the blot was cut into longitudinal strips, and each strip was probed with a di?erent antibody. Tracks contained the following MAbs: 1, normal mouse serum; 2, WU32 anti-HLA; 3. CT9-B7; 4, CT14-G4; S, Mycl-91510; 6, Mycl-8P9; 7, Mycl-3C7; 8. Mycl-6510; 9, Myb2-2.24. Prestained marker proteins were run in tracks alongside cell lysates, and molecular weights of detected antigens were estimated by comparison with the positions of these markers.

Specifications

Host Mouse
Clone 9E10
Isotype IgG1 kappa
Method of Purification cMyc antibody purified by affinity chromatography
Form & Buffer Supplied as liquid in PBS with 0.02% sodium azide
Concentration 1 mg/ml

Storage & Safety

Storage Store as concentrated solution. Aliquot and store at -20 deg C or below. Avoid repeated freeze-thaw cycles.

General Information

Biological Significance In addition to its role as a classical transcription factor, cMyc also functions to regulate global chromatin structure by regulating histone acetylation both in gene-rich regions and at sites far from any known gene.

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Images

  • Western Blot analysis using cMyc antibody (10-2499) | Immunoblotting assay of myc mAbs. COLO 320 HSR cells were dissolved in SDS buffer and fractionated on a 12.5% SDS-polyacrylamide gel. Fractionated proteins were electroblotted onto nitrocellulose paper, the blot was cut into longitudinal strips, and each strip was probed with a di?erent antibody. Tracks contained the following MAbs: 1, normal mouse serum; 2, WU32 anti-HLA; 3. CT9-B7; 4, CT14-G4; S, Mycl-91510; 6, Mycl-8P9; 7, Mycl-3C7; 8. Mycl-6510; 9, Myb2-2.24. Prestained marker proteins were run in tracks alongside cell lysates, and molecular weights of detected antigens were estimated by comparison with the positions of these markers.

Availability: In stock

Price: €350.10
Size: 250 ug
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