CNOT7 protein (His tag) (80R-1936)
Recombinant mouse CNOT7 protein
Overview
Overview
| Synonyms | CNOT7, CCR4-Not transcription complex protein, CNOT 7, subunit 7 protein, CNOT-7 protein, CAF-1 protein, CNOT-7, CNOT 7 protein |
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| Species | Mouse |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of CNOT7 protein (His tag) (80R-1936)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-248 amino acids: MGSSHHHHHH SSGLVPRGSH MGSMPAATVD HSQRICEVWA CNLDEEMKKI RQVIRKYNYV AMDTEFPGVV ARPIGEFRSN ADYQYQLLRC NVDLLKIIQL GLTFMNEQGE YPPGTSTWQF NFKFNLTEDM YAQDSIELLT TSGIQFKKHE EEGIETQYFA ELLMTSGVVL CEGVKWLSFH SGYDFGYLIK ILTNSNLPEE ELDFFEILRL FFPVIYDVKY LMKSCKNLKM FFEDHIDDAK YCGHLYGLGS GSSYVQNGTG NAYEEEASKQ S |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 80% pure |
| Molecular Weight | 31.1 kDa (271aa), confirmed by MALDI-TOF |
| Tag/Conjugate | His tag |
| Form & Buffer | Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT |
| Concentration | 1 mg/ml |
Usage & Assay Information
| Usage Recommendations | CNOT7 protein (His tag) has been used in SDS PAGE, and may be suitable for use in other assays, to be determined by the end user. |
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Storage & Safety
| Storage | Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles. |
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General Information
| Biological Significance | CNOT7, also known as CCR4-Not transcription complex, subunit 7, is a ubiquitous transcription factor. It is a component of the CCR4 complex which functions as a general transcription regulation complex. CNOT7 also binds to an anti-proliferative protein, B-cell translocation protein 1 (BTG1), which negatively regulates cell proliferation. Recombinant mouse CNOT7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
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