Cofilin 2 antibody (70R-12501)

Affinity purified Rabbit polyclonal Cofilin 2 antibody

Synonyms Polyclonal Cofilin 2 antibody, Anti-Cofilin 2 antibody, CFL2 antibody, Cofilin -2, Cofilin 2, Cofilin 2, cofilin-2 antibody, cofilin muscle isoform antibody, cofilin 2 (muscle) antibody, Cofilin -2 antibody, NEM7 antibody, Cofilin 2 antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB
Immunogen Cofilin 2 antibody was raised in rabbit using a synthetic peptide contain a sequence corresponding to a region within amino acids 92 and 142 of Cofilin 2

Images

Immunofluorescent staining using Cofilin 2 antibody (70R-12501)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cofilin 2 antibody at 1:200 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification Cofilin 2 antibody was purified by antigen-affinity chromatography
Molecular Weight 19 kDa
Form & Buffer Supplied as a concentrated soloution containing 1X PBS, 1% BSA, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:200, IHC-P: 1:100-1:250, ICC:1:100-1:200, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance CFL2 protein belongs to the actin-binding proteins ADF family that contains 1 ADF-H domain. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. Defects in CFL2 are the cause of nemaline myopathy type 7 (NEM7).

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using Cofilin 2 antibody (70R-12501)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cofilin 2 antibody at 1:200 dilution.
  • <div><font face=Calibri size=2>Immunohistochemical staining using Cofilin 2 antibody (70R-12501)</font></div>     | Immunohistochemical staining of paraffin-embedded gastric CA using Cofilin 2 antibody at a dilution of 1:100
  • <div><font face=Calibri size=2>Western blot analysis using Cofilin 2 antibody (70R-12501)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A:Raji) using a 12 % SDS PAGE gel and Cofilin 2 antibody at a dilution of 1:1000

Availability: In stock

Price: €323.11
Size: 100 ul
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