Collagen Type III antibody (70R-CR014)
Affinity purified Rabbit polyclonal Collagen Type III antibody
Overview
Overview
| Synonyms | Polyclonal Collagen Type III antibody, Anti-Collagen Type III antibody, Fetal collagen antibody, autosomal dominant antibody, Collagen type III alpha antibody, Collagen type III alpha 1 antibody, EDS4A antibody, Ehlers Danlos syndrome type IV |
|---|---|
| Specificity | Bovine/Human collagen type III |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, IHC, WB |
| Immunogen | Collagen type III antibody was raised in rabbit using collagen type III from human and bovine placenta as the immunogen. |
Images
Western Blot analysis using Collagen Type III antibody (70R-CR014)
Lane 1: Human Collagen III. Load: 100 ng per lane. Primary antibody: Collagen III antibody at 1:1000 ON at 4 deg C. Secondary antibody: Dylight (TM) 649 Goat anti-rabbit at 1:20, 000 for 30 min at RT. Block for 30 min at RT.
Specifications
| Host | Rabbit |
|---|---|
| Method of Purification | Collagen Type III antibody was purified by affinity chromatography. |
| Form & Buffer | Sterile filtered liquid in 0.125M Na2B4O7, pH 8.0, with 0.075M NaCl, 0.005M EDTA, and 0.01% NaN3. |
| Concentration | 2.0 mg/ml |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:5,000-1:10,000, IHC: 1:50-1:200, IP: 1:100, WB: 1:500-1:1,000 |
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| Bioactivity | Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues |
Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Collagen is a group of naturally occurring proteins found, in nature, exclusively in animals, especially in the flesh and connective tissues of mammals. It is the main component of connective tissue, and is the most abundant protein in mammals, making up about 25% to 35% of the whole-body protein content. Collagen alpha-1(III) chain is a protein that in humans is encoded by the COL3A1 gene, which is located on chromosome 2. Collagen alpha-1(III) chain is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I collagen. |
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