Cortactin antibody (70R-51531)

Purified Polyclonal Cortactin antibody

Synonyms Polyclonal Cortactin antibody, Anti-Cortactin antibody, EMS1 antibody, Src substrate cortactin antibody, Amplaxin antibody, Oncogene EMS1 antibody, CCTN antibody
Specificity Recognizes endogenous levels of Cortactin protein
Cross Reactivity Mouse
Applications IHC, IP, WB
Immunogen Cortactin antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cortactin (pY421) as the immunogen

Images

Western blot analysis using Cortactin antibody (70R-51531)

Western blot analysis of Cortactin (pY421) expression in HeLa UV-treated (A); NIH3T3 H2O2-treated (B) whole cell lysates.

Specifications

Host Rabbit
Method of Purification Cortactin antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IP: 1:10 - 1:100

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance Cortactin has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells.

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Images

  • 

Western blot analysis using Cortactin antibody (70R-51531)



 | 

Western blot analysis of Cortactin (pY421) expression in HeLa UV-treated (A); NIH3T3 H2O2-treated (B) whole cell lysates.
  • 

Immunohistochemical analysis using Cortactin antibody (70R-51531)



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Immunohistochemical analysis of Cortactin (pY421) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Availability: In stock

Price: €233.95
Size: 100 ul
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