Western blot analysis using Creatine kinase antibody (70R-12606)
Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 10 % SDS PAGE gel and Creatine kinase antibody at a dilution of 1:10000
Creatine kinase antibody
Western blot analysis using Creatine kinase antibody (70R-12606)
Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 10 % SDS PAGE gel and Creatine kinase antibody at a dilution of 1:10000
Western blot analysis using Creatine kinase antibody (70R-12606)
Western blot analysis of 30 ug of whole cell lysate (A:H1299; B:HeLa S3) using a 12 % SDS PAGE gel and Creatine kinase antibody at a dilution of 1:1000
Immunofluorescent staining using Creatine kinase antibody (70R-12606)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Creatine Kinase BB antibody at 1:100 dilution.
Immunohistochemical staining using Creatine kinase antibody (70R-12606)
Immunohistochemical staining of paraffin-embedded HSC3 xenograft using Creatine kinase antibody at a dilution of 1:100
Specifications
Host
Rabbit
Isotype
IgG
Method of Purification
Creatine kinase antibody was purified by antigen-affinity chromatography
Molecular Weight
43 kDa
Form & Buffer
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
General Information
Biological Significance
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.