Creatine kinase antibody (70R-12606)

Affinity purified Rabbit polyclonal Creatine kinase antibody

Synonyms Polyclonal Creatine kinase antibody, Anti-Creatine kinase antibody, creatine kinase B chain antibody, CKBB antibody, B-CK antibody, creatine kinase-B antibody, CKB antibody, creatine kinase B-type antibody
Cross Reactivity Human, Mouse
Applications ICC, IF, IHC-P, WB
Immunogen Creatine kinase antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 165 and 381 of Creatine kinase

Images

Western blot analysis using Creatine kinase antibody (70R-12606)

Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 10 % SDS PAGE gel and Creatine kinase antibody at a dilution of 1:10000

Specifications

Host Rabbit
Isotype IgG
Method of Purification Creatine kinase antibody was purified by antigen-affinity chromatography
Molecular Weight 43 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.54 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, IHC-P: 1:100-1:500, WB: 1:1000-1:10000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using Creatine kinase antibody (70R-12606)</font></div>     | Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 10 % SDS PAGE gel and Creatine kinase antibody at a dilution of 1:10000
  • <div><font face=Calibri size=2>Western blot analysis using Creatine kinase antibody (70R-12606)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A:H1299; B:HeLa S3) using a 12 % SDS PAGE gel and Creatine kinase antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunofluorescent staining using Creatine kinase antibody (70R-12606)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Creatine Kinase BB antibody at 1:100 dilution.
  • <div><font face=Calibri size=2>Immunohistochemical staining using Creatine kinase antibody (70R-12606)</font></div>     | Immunohistochemical staining of paraffin-embedded HSC3 xenograft using Creatine kinase antibody at a dilution of 1:100

Availability: In stock

Price: €323.11
Size: 100 ul
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