CRHR2 antibody (70R-49593)

Purified Polyclonal CRHR2 antibody

Synonyms Polyclonal CRHR2 antibody, Anti-CRHR2 antibody, CRF2R antibody, CRH2R antibody, Corticotropin-releasing factor receptor 2 antibody, CRF-R-2 antibody, CRF-R2 antibody, CRFR-2 antibody, Corticotropin-releasing hormone receptor 2 antibody, CRH-R-2 antibody, CRH-R2 antibody
Specificity Recognizes endogenous levels of CRHR2 protein
Cross Reactivity Mouse, Rat, Pig
Applications IHC, IP, WB
Immunogen CRHR2 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CRHR2 as the immunogen

Images

Western blot analysis using CRHR2 antibody (70R-49593)

Western blot analysis of CRHR2 expression in BC3H1 (A); U87MG (B) whole cell lysates.

Specifications

Host Rabbit
Method of Purification CRHR2 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IP: 1:10 - 1:100

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance CRHR2 belongs to the G-protein coupled receptor 2 family, and the subfamily of corticotropin releasing hormone receptor. This receptor shows high affinity for corticotropin releasing hormone (CRH), and also binds CRH-related peptides such as urocortin.

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Images

  • 

Western blot analysis using CRHR2 antibody (70R-49593)



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Western blot analysis of CRHR2 expression in BC3H1 (A); U87MG (B) whole cell lysates.
  • 

Immunohistochemical analysis using CRHR2 antibody (70R-49593)



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Immunohistochemical analysis of CRHR2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Availability: In stock

Price: €197.96
Size: 100 ul
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