CRP antibody (10-C33C)
Mouse monoclonal CRP antibody
|Synonyms||Monoclonal CRP antibody, Anti-CRP antibody, C-Reactive Protein antibody, C Reactive Protein antibody|
|Cross Reactivity||To be determined by end-user|
|Applications||ELISA, IHC, TBA|
|Immunogen||CRP antibody was raised in mouse using human CRP derived from pleural/ascetic fluid or plasma as the immunogen.|
|Assay Information||10-C33C can be used as the detection antibody in a matched pair immunoassay in conjunction with 10-C33A as the capture antibody|
|Method of Purification||CRP antibody was purified by chromatography on protein A Sepharose.|
|Form & Buffer||Protein A purified and supplied in PBS, pH 7.3, with 0.9% NaCl, and 0.1 % NaN3.|
|Concentration||Batch dependent - please inquire should you have specific requirements|
Usage & Assay Information
|Usage Recommendations||Optimal conditions to be determined by end user|
Storage & Safety
|Storage||Store at 4 deg C.|
|Biohazard Information||This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.|
|Biological Significance||C-reactive protein (CRP) is a protein found in the blood, the levels of which rise in response to inflammation (i.e. C-reactive protein is an acute-phase protein). Its physiological role is to bind to phosphocholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via the C1Q complex.|
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|Submitted By||Felipe Romero, Rice Univeristy|
|Date||13th February 2015|
|Application product used in||ELISA|
|Usage||Refer to comments|
|Comments||As suggested by Fitzgerald, for one of our sandwich immunoassays, we typically use CRP protein 30C-CP1000U with 10-C33A as the capturing antibody and 10-C33C as the detection antibody.
This combination works great because the binding is very strong and we are able to measure very low limits of detections. In the image provided, an array of agarose beads is used as a support for the sandwich immunoassay of CRP as captured by fluorescently labeled anti-CRP with Alexa Fluor 488.