CRYBB1 protein (His tag) (80R-1347)

Purified recombinant Human CRYBB1 protein

Synonyms CRYBB 1, Crystallin beta B1 protein, CRYBB 1 protein, Eye lens structural protein protein, CRYBB-1 protein, OTTHUMP00000028719., CRYBB 1 protein, CRYBB1 protein, CRYBB-1, CATCN3. protein, Beta-crystallin B1 Beta crystallin B1 protein, CRYBB1
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of CRYBB1 protein (His tag) (80R-1347)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-252 amino acids: MSQAAKASAS ATVAVNPGPD TKGKGAPPAG TSPSPGTTLA PTTVPITSAK AAELPPGNYR LVVFELENFQ GRRAEFSGEC SNLADRGFDR VRSIIVSAGP WVAFEQSNFR GEMFILEKGE YPRWNTWSSS YRSDRLMSFR PIKMDAQEHK ISLFEGANFK GNTIEIQGDD APSLWVYGFS DRVGSVKVSS GTWVGYQYPG YRGYQYLLEP GDFRHWNEWG AFQPQMQSLR RLRDKQWHLE GSFPVLATEP PKRSHHHHHH
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 29.1 kDa (260aa) confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol and 1mM DTT.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Crystallins are the major structural proteins of the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into alpha, beta, and gamma families. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. CRYBB1 is a beta basic group member and undergoes extensive cleavage at its N-terminal extension during lens maturation. Recombinant human CRYBB1 protein, fused to His-tag at C-terminus was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of CRYBB1 protein (His tag) (80R-1347) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €206.99
Size: 100 ug
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