CST6 protein (His tag) (80R-1900)
Purified recombinant CST6 protein
|Synonyms||Cystatin M protein, CST-6 protein, Cystatin E/M protein, Cystatin-E protein, CST-6, CST6, Cystatin-6 protein, CST 6 protein, CST 6|
Coomassie Blue stained SDS-PAGE of CST6 protein (His tag) (80R-1900)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||29-149 amino acids: MGSSHHHHHH SSGLVPRGSH MRPQERMVGE LRDLSPDDPQ VQKAAQAAVA SYNMGSNSIY YFRDTHIIKA QSQLVAGIKY FLTMEMGSTD CRKTRVTGDH VDLTTCPLAA GAQQEKLRCD FEVLVVPWQN SSQLLKHNCV QM|
|Grade & Purity||> 95% pure|
|Molecular Weight||15.9 kDa (142aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 1mM DTT and 0.1M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||CST6 is a member of the type 2 cystatin family. Some of the members are active cysteine protease inhibitors, while cystatin E/M moderate the inhibition of cathepsin B but is not active against cathepsin C. CST6 is a secretable protein that influences osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors, and the response to systemic inflammation. Recombinant human CST6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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