CTGF protein (30R-2206)

Purified recombinant Human CTGF protein

Synonyms Connective Tissue Growth Factor protein, CCN2 protein
Species Human
Protein Type Recombinant
Applications ELISA, Functional Assay
Assay Information This reagent has been tested by sandwich ELISA using the anti-mouse IL-1a ELISA pair (ALF-161/biotin-polyclonal) and in bioassays with an observed ED50 of 10 pg/ml, corresponding to a specific activity of 1.0 x 10E8 U/mg.

Specifications

Expression System E.coli
Grade & Purity > 98% pure
Method of Purification CTGF protein was purified by affinity chromatography.
Molecular Weight 11.2 kDa
Form & Buffer Supplied as a sterile liquid with phosphate buffered saline, pH 7.2,1.0% BSA. 0.22 um filtered.

Usage & Assay Information

Bioactivity The ED50, as determined by the dose-dependentinduction of HUVEC cell proliferation, is 1.6 ug/ml, corresponding to a specific activity of 6 x 10^2 Units/mg.

Storage & Safety

Storage Store at -70 deg C or less.
Endotoxin Levels Less than 0.01 ng/ug cytokine as determined by the LAL assay.

General Information

Biological Significance CTGF is a secreted protein produced by umbilical veins and vascular endothelial cells. CTGF possesses an Insulin-like Growth Factor -binding domain, a thrombospondin type 1 domain, and a cysteine knot region. CTGF plays important roles in the proliferation and differentiation of chondrocytes, induces angiogenesis, and promotes cell adhesion in fibroblasts, endothelial, and epithelial cells.

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Price From: Price: €188.14

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Price From: Price: €188.14
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