Recognizes endogenous levels of Cyclin A1/2 protein
Cross Reactivity
Mouse, Rat, Bovine, Chicken, Rabbit
Applications
ChIP, ICC, IF, IHC, WB
Immunogen
Cyclin A1/2 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cyclin A1/2 as the immunogen
Western blot analysis using Cyclin A1/2 antibody (70R-50654)
Western blot analysis of Cyclin A1/2 expression in HEK293T (A); NIH3T3 (B); rat brain (C); SW626 (D); SKOV3 (E); A2780 (F) whole cell lysates.
Cyclin A1/2 antibody
Western blot analysis using Cyclin A1/2 antibody (70R-50654)
Western blot analysis of Cyclin A1/2 expression in HEK293T (A); NIH3T3 (B); rat brain (C); SW626 (D); SKOV3 (E); A2780 (F) whole cell lysates.
Immunohistochemical analysis using Cyclin A1/2 antibody (70R-50654)
Immunohistochemical analysis of Cyclin A1/2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis using Cyclin A1/2 antibody (70R-50654)
Immunofluorescent analysis of Cyclin A1/2 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Specifications
Host
Rabbit
Method of Purification
Cyclin A1/2 antibody was purified by immunogen affinity chromatography
Form & Buffer
Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide
Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles
General Information
Biological Significance
Cyclin A1/2 belong to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell division cycle. Cyclins function as activating subunits of enzymatic complex together with cyclin-dependent kinases (CDKs).