Polyclonal Cyclophilin F antibody, Anti-Cyclophilin F antibody, CYP3 antibody, Peptidyl-prolyl cis-trans isomerase F mitochondrial antibody, PPIase F antibody, Cyclophilin D antibody, CyP-D antibody, CypD antibody, Cyclophilin F antibody, Mitochondrial cyclophilin antibody, CyP-M antibody, Rotamase F antibody
Specificity
Recognizes endogenous levels of Cyclophilin F protein
Cross Reactivity
Mouse, Rat, Rabbit
Applications
ICC, IF, IP, WB
Immunogen
Cyclophilin F antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cyclophilin F as the immunogen
Western blot analysis using Cyclophilin F antibody (70R-50773)
Western blot analysis of Cyclophilin F expression in HeLa (A); K562 (B); HL60 (C); Jurkat (D) whole cell lysates.
Cyclophilin F antibody
Western blot analysis using Cyclophilin F antibody (70R-50773)
Western blot analysis of Cyclophilin F expression in HeLa (A); K562 (B); HL60 (C); Jurkat (D) whole cell lysates.
Immunofluorescent analysis using Cyclophilin F antibody (70R-50773)
Immunofluorescent analysis of Cyclophilin F staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Specifications
Host
Rabbit
Method of Purification
Cyclophilin F antibody was purified by immunogen affinity chromatography
Form & Buffer
Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide
Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles
General Information
Biological Significance
Cyclophilin F catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated.