Cyclophilin F antibody (70R-50773)

Purified Polyclonal Cyclophilin F antibody

Synonyms Polyclonal Cyclophilin F antibody, Anti-Cyclophilin F antibody, CYP3 antibody, Peptidyl-prolyl cis-trans isomerase F mitochondrial antibody, PPIase F antibody, Cyclophilin D antibody, CyP-D antibody, CypD antibody, Cyclophilin F antibody, Mitochondrial cyclophilin antibody, CyP-M antibody, Rotamase F antibody
Specificity Recognizes endogenous levels of Cyclophilin F protein
Cross Reactivity Mouse, Rat, Rabbit
Applications ICC, IF, IP, WB
Immunogen Cyclophilin F antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cyclophilin F as the immunogen

Images

Western blot analysis using Cyclophilin F antibody (70R-50773)

Western blot analysis of Cyclophilin F expression in HeLa (A); K562 (B); HL60 (C); Jurkat (D) whole cell lysates.

Specifications

Host Rabbit
Method of Purification Cyclophilin F antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IF: 1:100 - 1:500, ICC: 1:100 - 1:500, IP: 1:10 - 1:100

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance Cyclophilin F catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated.

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Images

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Western blot analysis using Cyclophilin F antibody (70R-50773)



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Western blot analysis of Cyclophilin F expression in HeLa (A); K562 (B); HL60 (C); Jurkat (D) whole cell lysates.
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Immunofluorescent analysis using Cyclophilin F antibody (70R-50773)



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Immunofluorescent analysis of Cyclophilin F staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Price: €197.96
Size: 100 ul
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