CYR61 antibody (10R-1099)

Mouse monoclonal CYR61 antibody

Synonyms Monoclonal CYR61 antibody, Anti-CYR61 antibody, CCN family member 1 antibody, IBP-10 antibody, CYR -61 antibody, CYR61, cysteine-rich angiogenic inducer 61 antibody, CYR 61 antibody, CCN1 antibody, CYR -61, CYR 61, GIG1 antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, IHC, WB
Immunogen CYR61 antibody was raised in mouse using recombinant human CYR61 (168-242aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of CYR61 antibody (10R-1099)

The cell lysates of Jurkat (40 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human CYR61 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone 3H3
Isotype IgG1 kappa
Source Anti-human CYR61 antibody, clone 3H3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CYR61 protein.
Method of Purification CYR61 antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IHC: 1:50-1:100, WB: 1:250-1:1,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Cysteine-rich angiogenic inducer 61, also known as CYR61, is a growth factor-inducible, immediate-early gene that has multifaceted activities in various cancers. CYR61 is a secreted heparin-binding extracellular matrix-associated protein whose activities include the promotion of adhesion and chemotaxis, and the stimulation of fibroblast and endothelial cell growth. CYR61 induces angiogenesis and promotes tumor growth and is expressed in dermal fibroblasts during cutaneous wound healing.

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Images

  • Western Blot analysis of CYR61 antibody (10R-1099) | The cell lysates of Jurkat (40 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human CYR61 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: €277.55
Size: 100 ul
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