Polyclonal Cytochrome c antibody, Anti-Cytochrome c antibody, cyt c antibody, Cytochrome complexantibody, Cytochrome-c antibody
Applications
ICC, IHC-F, IHC-P, WB
Immunogen
Cytochrome c antibody was raised in Rabbit using a synthetic peptide corresponding to a sequence at the C-terminal of human Cytochrome C, identical to the related Rabbit and rat sequence as the immunogen
Immunohistochemical staining using Cytochrome c antibody (70R-13781)
Immunohistochemistry of paraffin-embedded section of Rat Lung Tissue
Cytochrome c antibody
Immunohistochemical staining using Cytochrome c antibody (70R-13781)
Immunohistochemistry of paraffin-embedded section of Rat Lung Tissue
Western Blot analysis using Cytochrome c antibody (70R-13781)
Lane 1: Recombinant Human Cytochrome C Protein 10ng; Lane 2: Recombinant Human Cytochrome C Protein 5ng; Lane 3: Recombinant Human Cytochrome C Protein 2.5ng; Lane 4: Jurkat Cell Lysate
Specifications
Host
Rabbit
Isotype
IgG
Method of Purification
Cytochrome c antibody was purified by affinity chromatography
Optimal conditions to be determined by the end user
Storage & Safety
Storage
Store at 4 deg C for short term storage. For long term storage aliquot and store at -20 deg C, avoid repeated freeze/thaw cycles.
General Information
Biological Significance
Cytochrome c (MW 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic Cytochrome c is associated with Apaf 1 and Caspase 9 to activate Caspase 3 and other caspases.