DACT3 antibody (10R-1123)

Mouse monoclonal DACT3 antibody

Synonyms Monoclonal DACT3 antibody, Anti-DACT3 antibody, DACT3, DACT 3 antibody, dapper antagonist of beta-catenin homolog 3 (Xenopus laevis) antibody, DACT 3, arginine rich region 1 antibody, DACT -3 antibody, thymus expressed gene 3-like antibody, DACT -3, MGC15476 antibody
Specificity Human
Cross Reactivity Human, Mouse
Applications ELISA, IHC, WB
Immunogen DACT3 antibody was raised in mouse using recombinant human DACT3 (89-202aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of DACT3 antibody (10R-1123)

Cell lysates of mouse brain and 293T (50 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human DACT3 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone 2A5
Isotype IgG1 kappa
Source Anti-human DACT3 antibody, clone 2A5, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human DACT3 protein.
Method of Purification DACT3 antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:250-1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance DACT3, a member of the DACT (Dpr/Frodo) gene family, plays an important role as a negative regulator of Wnt/-catenin signaling. Unlike other Wnt signaling inhibitors that are silenced by DNA methylation, DACT3 repression is associated with bivalent histone modifications.

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Images

  • Western Blot analysis of DACT3 antibody (10R-1123) | Cell lysates of mouse brain and 293T (50 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human DACT3 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: €249.33
Size: 100 ul
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