DAP5 antibody (70R-12601)

Affinity purified Rabbit polyclonal DAP5 antibody

Synonyms Polyclonal DAP5 antibody, Anti-DAP5 antibody, eukaryotic translation initiation factor 4 gamma 2 antibody, DAP 5 antibody, FLJ41344 antibody, DAP-5, AAG1 antibody, DAP5 antibody, EIF4G2 antibody, eIF-4G 2 antibody, DAP-5 antibody, aging-associated protein 1 antibody, P97 antibody, eukaryotic translation initiation factor 4G-like 1 antibody, DAP-5 antibody, DAP 5, DAP5, death-associated protein 5 antibody, NAT1 antibody
Cross Reactivity Human, Mouse
Applications IHC-P, WB
Immunogen DAP5 antibody was raised in rabbit using a recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 194 of DAP5

Images

Western blot analysis using DAP5 antibody (70R-12601)

Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 5 % SDS PAGE gel and DAP5 antibody at a dilution of 1:500

Specifications

Host Rabbit
Isotype IgG
Method of Purification DAP5 antibody was purified by antigen-affinity chromatography
Molecular Weight 102 kDa
Form & Buffer Supplied as a concentrated soloution containing 1X PBS, 40% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using DAP5 antibody (70R-12601)</font></div>     | Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 5 % SDS PAGE gel and DAP5 antibody at a dilution of 1:500
  • <div><font face=Calibri size=2>Immunohistochemical staining using DAP5 antibody (70R-12601)</font></div>     | Immunohistochemical staining of paraffin-embedded Skeletal Muscle using DAP5 antibody
  • <div><font face=Calibri size=2>Western blot analysis using DAP5 antibody (70R-12601)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A:A431) using a 7.5 % SDS PAGE gel and DAP5 antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunohistochemical staining using DAP5 antibody (70R-12601)</font></div>     | Immunohistochemical staining of paraffin-embedded Cal27 xenograft using DAP5 antibody at a dilution of 1:100

Availability: In stock

Price: €323.11
Size: 100 ul
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