DDIT4 protein (His tag) (80R-1654)
Purified recombinant Human DDIT4 protein
|Synonyms||DNA damage-inducible transcript 4 protein protein, DDIT-4, REDD1 protein, DDIT-4 protein, RP11-442H21.1 protein, RTP801, DDIT 4, Dig2 protein, FLJ20500 protein, DDIT 4 protein, DDIT4|
Coomassie Blue stained SDS-PAGE of DDIT4 protein (His tag) (80R-1654)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-232 amino acids: MGSSHHHHHH SSGLVPRGSH MPSLWDRFSS SSTSSSPSSL PRTPTPDRPP RSAWGSATRE EGFDRSTSLE SSDCESLDSS NSGFGPEEDT AYLDGVSLPD FELLSDPEDE HLCANLMQLL QESLAQARLG SRRPARLLMP SQLVSQVGKE LLRLAYSEPC GLRGALLDVC VEQGKSCHSV GQLALDPSLV PTFQLTLVLR LDSRLWPKIQ GLFSSANSPF LPGFSQSLTL STGFRVIKKK LYSSEQLLIE EC|
|Grade & Purity||> 90% pure|
|Molecular Weight||27.5 kDa (252aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 0.2M NaCl, 5mM DTT, 1mM EDTA and 30% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||DDIT4, also known as Dig2 or REDD1, is thought to function in the regulation of reactive oxygen species. In response to stress due to DNA damage and glucocorticoid treatment, DDIT4 is upregulated at the transcriptional level. DDIT4 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. Recombinant human DDIT4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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