DEDD antibody (70R-13451)

Affinity purified Rabbit polyclonal DEDD antibody

Synonyms Polyclonal DEDD antibody, Anti-DEDD antibody, CASP8IP1 antibody, DEFT antibody, DEDD antibody, death effector domain-containing testicular molecule antibody, FLDED-1 antibody, DEDD1 antibody, FLDED1 antibody
Cross Reactivity Human
Applications ICC, IF, WB

Images

Immunofluorescent staining using DEDD antibody (70R-13451)

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DEDD antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.

Specifications

Host Rabbit
Isotype IgG
Method of Purification DEDD antibody was purified by antigen-affinity chromatography
Molecular Weight 37 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.42 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:1000, ICC:1:100-1:1000, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance This gene encodes a protein that contains a death effector domain. DED is a protein-protein interaction domain shared by adaptors, regulators and executors of the programmed cell death pathway. Overexpression of this gene was shown to induce weak apoptosis. Upon stimulation, this protein was found to translocate from cytoplasm to nucleus and colocalize with UBTF, a basal factor required for RNA polymerase I transcription, in the nucleolus.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using DEDD antibody (70R-13451)</font></div>     | Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DEDD antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.
  • <div><font face=Calibri size=2>Western blot analysis using DEDD antibody (70R-13451)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: Raji) using a 10 % SDS PAGE gel and DEDD antibody at a dilution of 1:1000

Availability: In stock

Price: €323.11
Size: 100 ul
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