Immunofluorescent staining using DEDD antibody (70R-13451)
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DEDD antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.
DEDD antibody
Immunofluorescent staining using DEDD antibody (70R-13451)
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DEDD antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.
Western blot analysis using DEDD antibody (70R-13451)
Western blot analysis of 30 ug of whole cell lysate (A: Raji) using a 10 % SDS PAGE gel and DEDD antibody at a dilution of 1:1000
Specifications
Host
Rabbit
Isotype
IgG
Method of Purification
DEDD antibody was purified by antigen-affinity chromatography
Molecular Weight
37 kDa
Form & Buffer
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
General Information
Biological Significance
This gene encodes a protein that contains a death effector domain. DED is a protein-protein interaction domain shared by adaptors, regulators and executors of the programmed cell death pathway. Overexpression of this gene was shown to induce weak apoptosis. Upon stimulation, this protein was found to translocate from cytoplasm to nucleus and colocalize with UBTF, a basal factor required for RNA polymerase I transcription, in the nucleolus.