DENV protein (His tag) (80R-3888)
Purified recombinant DENV protein (His tag)
Overview
Overview
| Synonyms | Dengue virus 2 envelope protein protein, protein |
|---|---|
| Species | Viral |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
SDS Page of DENV protein (His tag) (80R-3888)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 298-400 aa: MGSSHHHHHH SSGLVPRGSH MGSSYSMCTG KFKVVKEIAE TQHGTIVIRV QYEGDGSPCK IPFEIMDLEK RHVLGRLITV NPIVTEKDSP VNIEAEPPFG DSYIIIGVEP GQLKLNWFKK GSSIGQ |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Method of Purification | DENV protein was purified by proprietary chromatographic techniques |
| Molecular Weight | 13.8 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT |
| Concentration | 500 ug/ml |
Storage & Safety
| Storage | Can be stored at 4 deg C short term. For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles |
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General Information
| Biological Significance | Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. This protein is synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes. |
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