Immunofluorescent staining using Desmoglein 2 antibody (70R-12672)
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Desmoglein 2 antibody at 1:500 dilution.
Desmoglein 2 antibody
Immunofluorescent staining using Desmoglein 2 antibody (70R-12672)
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Desmoglein 2 antibody at 1:500 dilution.
Immunohistochemical staining using Desmoglein 2 antibody (70R-12672)
Immunohistochemical staining of paraffin-embedded DU145 xenograft using Desmoglein 2 antibody at a dilution of 1:100
Western blot analysis using Desmoglein 2 antibody (70R-12672)
Western blot analysis of 30 ug of whole cell lysate (A: 293T; B: H1299; C: Molt-4) using a 7.5 % SDS PAGE gel and Desmoglein 2 antibody at a dilution of 1:1000
Specifications
Host
Rabbit
Isotype
IgG
Method of Purification
Desmoglein 2 antibody was purified by antigen-affinity chromatography
Molecular Weight
122 kDa
Form & Buffer
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
General Information
Biological Significance
Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. This gene product is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines.