DHEA Sulphate Elisa Kit (55R-RE52181)
ELISA kit for detection of DHEA Sulphate in the research laboratory
Overview
Overview
| Synonyms | DHEA-S Elisa Kit, DHEAS ELISA Kit, Dehydroepiandrosterone Sulfate ELISA Kit |
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| Applications | ELISA |
| Assay Information | The DHEA-S ELISA Kit is a solid phase ELISA, based on the principle of competitive binding. The microtiter wells are coated with a polyclonal antibody directed towards a unique antigenic site of the DHEA-S molecule. Endogenous DHEA-S of a patient sample competes with a DHEA-S horseradish peoxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is reverse proportional to the concentration of DHEA-S in the sample. After addition of the the substrate solution, the intensity of colour developed is reverse proportional to the concentration of DHEA-S in the patient sample. |
Usage & Assay Information
| Usage Recommendations | Optimal conditions to be determined by end user |
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Storage & Safety
| Storage | Store at 4 deg C. |
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| Biohazard Information | All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV I/II, HbsAg and HCV. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as |
General Information
| Biological Significance | Dehydroepiandrosterone (DHEA) is a steroid hormone present in blood mostly in its sulfate form (DHEA-S). DHEA-S is a more specific product of the adrenals and measurements of this steroid are widely used in clinical practice. The clinical importance of plasma assays of DHEA-S is associated with the diagnosis of adrenal hyperplasia and differential diagnosis of hirsutism. |
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