Diablo antibody (20R-2899)
Rabbit polyclonal Diablo antibody
Overview
Overview
| Synonyms | Polyclonal Diablo antibody, Anti-Diablo antibody, DIABLO antibody, diablo antibody, IAP-binding mitochondrial protein antibody, BOS_16495 antibody, DBO antibody, DFNA64 antibody, diablo homolog antibody, diablo homolog (Drosophila) antibody, diablo homolog antibody, mitochondrial antibody, DIABLO-S antibody, direct IAP binding protein with low PI antibody, Direct IAP-binding protein with low pI antibody, hCG_1782202 antibody, mitochondrial diablo antibody, mitochondrial diablo-like protein antibody, mitochondrial Smac protein antibody, Second mitochondria-derived activator of caspase antibody, second mitochondrial derived activator of caspases antibody, Smac antibody, smac3 antibody, XSmac antibody |
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| Cross Reactivity | Human |
| Applications | IHC-P, WB |
| Immunogen | Diablo antibody was raised in Rabbit using a synthetic target peptide as the immunogen |
Specifications
| Host | Rabbit |
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| Isotype | IgG |
| Method of Purification | Diablo antibody was purified by Protein A affinity chromatography |
| Form & Buffer | Supplied in liquid form in HEPES buffer with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol |
Usage & Assay Information
| Usage Recommendations | WB: 1:1000-2000 |
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Storage & Safety
| Storage | Aliquot and store for 1 year at -20 deg C, avoid freeze/thaw cycles |
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General Information
| Biological Significance | The mitochondrial protein Smac/DIABLO (second mitochondria-derived activator) performs a critical function in apoptosis by eliminating the inhibitory effect of IAPs (inhibitor of apoptosis proteins) on caspases. The newly synthesized Smac protein contains 239 amino acids. Its N-terminal 55 residues encode the mitochondrial-targeting sequence and are proteolytically removed in the mature Smac protein. In the intrinsic cell death pathway, the key event leading to the activation of caspases is the release of several pro-apoptotic proteins such as Smac/DIABLO from the intermembrane space of mitochondria into the cytosol. During apoptosis, Smac is released from mitochondria and re-activates the processed initiator and effector caspases by relieving IAP-mediated inhibition. Furthermore, Smac/DIABLO plays an important regulatory role in the sensitization of cancer cells to both immune-and drug-induced apoptosis. |
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