DIAPH1 antibody (70R-12637)

Affinity purified Rabbit polyclonal DIAPH1 antibody

Synonyms Polyclonal DIAPH1 antibody, Anti-DIAPH1 antibody, DFNA1 antibody, DIAPH-1, DIAPH 1, DIAPH-1 antibody, diaphanous-related formin-1 antibody, FLJ25265 antibody, DRF1 antibody, hDIA1 antibody, DIAPH1 antibody, DIAPH1, DIAPH 1 antibody, protein diaphanous homolog 1 antibody, DIA1 antibody, LFHL1 antibody, diaphanous-related formin 1 antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB
Immunogen DIAPH1 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 1 and 237 of DIAPH1

Images

Immunofluorescent staining using DIAPH1 antibody (70R-12637)

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DIAPH1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.

Specifications

Host Rabbit
Isotype IgG
Method of Purification DIAPH1 antibody was purified by antigen-affinity chromatography
Molecular Weight 141 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.69 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:1000, ICC:1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using DIAPH1 antibody (70R-12637)</font></div>     | Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DIAPH1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
  • <div><font face=Calibri size=2>Immunohistochemical staining using DIAPH1 antibody (70R-12637)</font></div>     | Immunohistochemical staining of paraffin-embedded gastric tissue using DIAPH1 antibody at a dilution of 1:100
  • <div><font face=Calibri size=2>Western blot analysis using DIAPH1 antibody (70R-12637)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: H1299; B: Raji) using a 7.5 % SDS PAGE gel and DIAPH1 antibody at a dilution of 1:1000

Availability: In stock

Price: €323.11
Size: 100 ul
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