Dihydrotestosterone ELISA Kit (55R-DB52021)
ELISA kit for detection of Dihydrotestosterone in the research laboratory
Overview
Overview
| Synonyms | DHT ELISA kit, Testosterone ELISA kit |
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| Specificity | 100 % dihydrotestosterone |
| Cross Reactivity | Numerous other steroids tested for cross reactivity. See datasheet. |
| Applications | ELISA |
| Assay Information | The sensitivity of the Direct DihydrotestosteroneELISA kit is 6.0 pg/ml. |
Usage & Assay Information
| Usage Recommendations | Optimal conditions to be determined by end user |
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Storage & Safety
| Storage | Store at 4 deg C. |
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| Biohazard Information | All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV, HbsAg and HCV. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as pote |
General Information
| Product Use | The following compounds were tested for cross-reactivity with the Direct Dihydrotestosterone ELISA kit with dihydrotestosterone cross-reacting at 100%; Testosterone 8.7%, 5 beta Dihydrotestosterone 2.0%, Androstenedione 0.2%. The following steroids cross-reacted at less than 0.01%: Dehydroepiandrosterone Sulfate, 17 beta-Estradiol, Estriol, Estrone, Progesterone, 17-OH Progesterone, Cortisol, and Pregnenolone. |
|---|---|
| Biological Significance | 5 alpha-dihydrotestosterone (DHT) is a steroid similar to testosterone and androstenedione, which belong to a class called androgens. DHT is a C19 steroid and possesses androgenic activity. DHT has at least three times the binding affinity for sex hormone binding globulin (SHBG) than testosterone. In males about 70% of DHT is derived from peripheral conversion of testosterone, while in females most of the DHT is derived from androstenedione. |
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