DKK1 protein (30R-AD010)
Human recombinant DKK1 protein
Overview
Overview
| Synonyms | Dickkopf-related protein-1 protein, DKK 1, Dickkopt-1 protein, SK protein, DKK-1 protein, DKK-1, DKK-1 protein, DKK 1 protein, DKK1 |
|---|---|
| Species | Human |
| Protein Type | Recombinant |
| Applications | User optimized |
Specifications
| Residues | Region of DKK1 protein corresponding to amino acids TLNSVLNSNA IKNLPPPLGG AAGHPGSAVS AAPGILYPGG NKYQTIDNYQ PYPCAEDEEC GTDEYCASPT RGGDAGVQIC LACRKRRKRC MRHAMCCPGN YCKNGICVSS DQNHFRGEIE ETITESFGND HSTLDGYSRR TTLSSKMYHT KGQEGSVCLR SSDCASGLCC ARHFWSKICK PVLKEGQVCT KHRRKGSHGL EIFQRCYCGE GLSCRIQKDH HQASNSSRLH TCQRH. |
|---|---|
| Expression System | 293 cells |
| Grade & Purity | > 97% pure |
| Molecular Weight | 35-40 kDa |
| Form & Buffer | Lyophilized from PBS buffer, pH 7.4 |
Usage & Assay Information
| Bioactivity | Determined by its ability to inhibit mWnt3a-induced TCF/LEF-luciferase activity in reporter HEK293 cells. |
|---|
Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
|---|---|
| Endotoxin Levels | Endotoxin level is less than 0.1 ng per ug (1 EU/ug). |
General Information
| Biological Significance | DKK-1 is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally identified as a Xenopus head forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 and DKK-4 play an important regulatory role in the Wnt /beta-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/betacatenin signaling system. LPR5 and LPR6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/betacatenin signaling cascade. It has been suggested that by inhibiting Wnt/beta-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis. |
|---|
Reviews
You need to be logged in to write a review. Please login here
Sorry there are currently no reviews for this product