DKK3 antibody (70R-12543)

Affinity purified Rabbit polyclonal DKK3 antibody

Synonyms Polyclonal DKK3 antibody, Anti-DKK3 antibody, hDkk-3 antibody, dkk-3 antibody, dickkopf-3 antibody, REIC antibody, DKK3 antibody, DKK-3, dickkopf homolog 3 (Xenopus laevis) antibody, DKK 3 antibody, DKK3, DKK-3 antibody, dickkopf-related protein 3 antibody, regulated in glioma antibody, DKK 3, RIG-like 7-1 antibody, dickkopf homolog 3 antibody, RIG antibody, RIG-like 5-6 antibody
Cross Reactivity Human, Mouse
Applications ICC, IF, WB
Immunogen DKK3 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 6 and 343 of DKK3

Images

Western blot analysis using DKK3 antibody (70R-12543)

Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 10 % SDS PAGE gel and DKK3 antibody at a dilution of 1:1000

Specifications

Host Rabbit
Isotype IgG
Method of Purification DKK3 antibody was purified by antigen-affinity chromatography
Molecular Weight 38 kDa
Form & Buffer Supplied as a concentrated soloution containing 1X PBS, 1% BSA, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using DKK3 antibody (70R-12543)</font></div>     | Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 10 % SDS PAGE gel and DKK3 antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunofluorescent staining using DKK3 antibody (70R-12543)</font></div>     | Immunofluorescence analysis of methanol-fixed A549, using DKK3 antibody at 1:500 dilution.
  • <div><font face=Calibri size=2>Western blot analysis using DKK3 antibody (70R-12543)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: A549) using a 10 % SDS PAGE gel and DKK3 antibody at a dilution of 1:1000

Availability: In stock

Price: €323.11
Size: 100 ul
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