Western Blot analysis of immunized recombinant protein using DMTF1 antibody (10R-1205)
DMTF1 antibody
Western Blot analysis of immunized recombinant protein using DMTF1 antibody (10R-1205)
Specifications
Host
Mouse
Clone
DMTF5I250
Isotype
IgG1
Method of Purification
DMTF1 antibody was purified using protein G column chromatography from culture supernatant of hybridoma
cultured in a medium containing bovine IgG-depleted
(approximately 95%) fetal bovine serum.
Form & Buffer
DMTF1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).
Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information
This antibody solution contains sodium azide (NaN )
as a preservative. There is a potential hazard that
NaN reacts with copper or lead to produce an explosive
compound. For safe disposal, the vial has to
be washed thoroughly with water. It is strongly adv
General Information
Biological Significance
This gene encodes a transcription factor that contains a cyclin D-binding domain, three central Myb-like repeats, and
two flanking acidic transactivation domains at the N- and C-termini. The encoded protein is induced by the oncogenic
Ras signaling pathway and functions as a tumor suppressor by activating the transcription of ARF and thus the ARF-p53
pathway to arrest cell growth or induce apoptosis. It also activates the transcription of aminopeptidase N and may
play a role in hematopoietic cell differentiation. The transcriptional activity of this protein is regulated by
binding of D-cyclins. This gene is hemizygously deleted in approximately 40% of human non-small-cell lung cancer and
is a potential prognostic and gene-therapy target for non-small-cell lung cancer. Multiple transcript variants
encoding different isoforms have been found for this gene.