DnaJ protein (30R-1024)
Purified recombinant E.coli DnaJ protein
|Synonyms||HSPF4 protein, , DnaJ homolog subfamily A member 1 protein, HDJ-2 protein, DnaJ (1-376aa) protein, HDJ2 protein, DnaJ protein homolog 2 protein, DNAJA1 protein, HSJ2 protein, HSJ-2 protein, Heat shock 40 kDa protein 4 protein, HSDJ protein, DNAJ2 protein|
Coomassie Blue stained SDS-PAGE of DnaJ protein (30R-1024)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-376 amino acids: MAKQDYYEIL GVSKTAEEHE IRKAYKRLAM KYHPDRNQGD KEAEAKFKEI KEAYEVLTDS QKRAAYDQYG HAAFEQGGMG GGGFGGGADF SDIFGDVFGD IFGGGRGRQR AARGADLRYN MELTLEEAVR GVTKEIRIPT LEECDVCHGS GAKPGTQPQT CPTCHGSGQV QMRQGFFAVQ QTCPHCQGRG TLIKDPCNKC HGHGRVERSK TLSVKIPAGV DTGDRIRLAG EGEAGEHGAP AGDLYVQVQV KQHPIFEREG NNLYCEVPIN FAMAALGGEI EVPTLDGRVK LKVPGETQTG KLFRMRGKGV KSVRGGAQGD LLCRVVVETP VGLNERQKQL LQELQESFGG PTGEHNSPRS KSFFDGVKKF FDDLTR|
|Grade & Purity||> 95% pure|
|Molecular Weight||41.1 kDa (376aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 25mM Tris-HCl, pH 7.5, containing 100 mM NaCl, 5mM DTT, and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||DNAJ, Heat shock protein, functions in association with DnaK(Hsp70) molecular chaperone to facilitate protein folding. p70 chaperone. DnaJ plays a key role in the chaperone reaction by stimulating the ATPase activity and activating the substrate binding of Hsp70. DnaJ consists of four domains that are N-terminal 76 amino acid J-domain, G/F domain, zinc-binding cystein rich CR-domain, C-terminal CTD-domain and they are conserved to various degrees among the homologues. DnaJ(amino acids 1-376) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques.|
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