Dnak protein (30R-1023)
Purified recombinant E.coli Dnak protein
|Synonyms||Dnak (508-638) protein, Heat shock protein 70 protein, HSP70 protein, A lid covering the substrate protein, Chaperone Hsp 70 protein, , Heat shock 70 kDa protein protein, Heat shock 70 kDa protein protein, dnaK protein, Co chaperone with DnaJ protein, Chaperone protein dnaK protein|
Coomassie Blue stained SDS-PAGE of Dnak protein (30R-1023)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||A lid covering the substrate 508-638 amino acids: MNEDEIQKMV RDAEANAEAD RKFEELVQTR NQGDHLLHST RKQVEEAGDK LPADDKTAIE SALTALETAL KGEDKAAIEA KMQELAQVSQ KLMEIAQQQH AQQQTAGADA SANNAKDDDV VDAEFEEVKD KK|
|Grade & Purity||> 95% pure|
|Molecular Weight||14.6 kDa (132aa)|
|Form & Buffer||Supplied as a liquid in 25mM Tris-HCl, pH 7.5, containing 100 mM NaCl, 1mM DTT, and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||DNAK, originally identified for its DNA replication by bacteriophage in E. coli is the bacterial hsp70 chaperone. The protein coding region of the substrate binding domain of DNAK (amino acids 385-546) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid(Met) is attached at N- terminus.|
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