DNAL4 protein (His tag) (80R-1822)
Purified recombinant Human DNAL4 protein
|Synonyms||DNAL 4 protein, DNAL-4 protein, Dynein light chain 4 protein, DNAL4, DNAL-4, DNAL 4, PIG27 protein, axonemal protein|
Coomassie Blue stained SDS-PAGE of DNAL4 protein (His tag) (80R-1822)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-105 amino acids: MGSSHHHHHH SSGLVPRGSH MGETEGKKDE ADYKRLQTFP LVRHSDMPEE MRVETMELCV TACEKFSNNN ESAAKMIKET MDKKFGSSWH VVIGEGFGFE ITHEVKNLLY LYFGGTLAVC VWKCS|
|Grade & Purity||> 95% pure|
|Molecular Weight||14.1 kDa (125aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 0.1M NaCl and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||DNAL4 is a component of the dynein motor complex in cells which converts the chemical energy contained in ATP into the mechanical energy of movement. Dynein transports various cellular cargo by 'walking' along cytoskeletal microtubules towards the minus-end of the microtubule, which is usually oriented towards the cell center. Recombinant human DNAL4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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