Donkey anti-Mouse IgG (H+L) biotin (43C-CB1526)
Donkey ant-mouse IgG (H + L) secondary antibody biotin
Overview
Overview
| Synonyms | Donkey anti-Mouse Immunoglobulin G (H + L) secondary antibody biotin |
|---|---|
| Specificity | Mouse IgG |
| Cross Reactivity | A single precipitin arc was observed against anti - Biotin, anti - Donkey serum, Mouse IgG and Mouse serum, when assayed by Immunoelectrophoresis. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and S |
| Applications | ELISA, IHC, WB |
| Immunogen | Mouse IgG whole molecule |
Specifications
| Host | Donkey |
|---|---|
| Tag/Conjugate | Biotin |
| Form & Buffer | Supplied lyophilized in 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.2, with 10 mg/ml BSA (IgG and Protease free), and 0.01% Sodium Azide as a preservative. Reconstitute with 1.0 ml of deionized water. |
| Concentration | 1.0mg/ml (by UV absorbance at 280 nm) after reconstitution. |
Usage & Assay Information
| Usage Recommendations | ELISA 1:20,000 - 1:100,000 WB 1:2,000 - 1:10,000 IHC 1:1,000 - 1:5,000 |
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Storage & Safety
| Storage | Store at 4 deg C prior to reconstitution. For longer storage after reconstitution, mix with 50% glycerol, aliquot and freeze at -20 deg C. |
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General Information
| Biological Significance |
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