Donkey anti Rat IgG (H + L) (HRP) (43R-ID068HRP)
Donkey anti Rat IgG (H + L) secondary antibody (HRP)
Overview
Overview
| Synonyms | Donkey anti Rat Immunoglobulin G (H + L) secondary antibody (HRP) |
|---|---|
| Specificity | This antibody reacts with heavy (gamma) chains on rat IgG and light chains on all rat immunoglobulins |
| Cross Reactivity | No reactivity is observed to non-immunoglobulin rat serum proteins or IgG from bovine, chicken, Goat, Guinea Pig, hamster, horse, human, mouse, rabbit or sheep. |
| Applications | User optimized |
| Immunogen | Donkey anti-Rat IgG (H + L) (HRP) was raised in donkey using purified Rat IgG (H&L) as the immunogen. |
Specifications
| Host | Donkey |
|---|---|
| Source | Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. |
| Method of Purification | Affinity purified using solid phase Rat IgG (H&L) |
| Tag/Conjugate | HRP |
| Form & Buffer | Lyophilized powder containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.1% (v/v) Kathon CG. Protease/IgG free, pH 7.2. Rehydrate with 1.1 ml of deionized water. Prepare fresh working dilution daily. |
Usage & Assay Information
| Usage Recommendations | 1:200-1:5000 ELISA/WB, 1:500-1:5000 IHC |
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Storage & Safety
| Storage | Store lyophilized material at 2-8 deg C. |
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General Information
| Biological Significance | Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. IgG constitutes 75% of serum immunoglobulins in humans. IgG molecules are synthesized and secreted by plasma B cells. |
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