Donkey anti Sheep IgG (H + L) (biotin) (43R-ID025bt)
Donkey anti Sheep IgG (H + L) secondary antibody (biotin)
Overview
Overview
| Synonyms | Donkey anti Sheep Immunoglobulin G (H + L) secondary antibody (biotin) |
|---|---|
| Specificity | Sheep |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, FC, ICC, IHC, WB |
| Immunogen | Donkey anti-sheep IgG (H + L) (biotin) was raised in donkey using sheep IgG (H&L) as the immunogen. |
Specifications
| Host | Donkey |
|---|---|
| Method of Purification | Donkey anti Sheep IgG (H + L) (bioton) was purified by affinity chromatography. |
| Tag/Conjugate | Biotin |
| Form & Buffer | Lyophilized from 0.01M Na3PO4, 0.25M NaCl, pH 7.6, with 15mg/ml BSA, and 0.05% NaN3. Reconstitute with 0.5 ml of distilled Water. |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:20,000-1:400,000, FC: 1:200-1:1,000, ICC: 1:500-1:5,000, IHC: 1:500-1:5,000, WB: 1:20,000-1:400,000 |
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Storage & Safety
| Storage | Store at 4 deg C. |
|---|---|
| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling. |
General Information
| Biological Significance | Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. IgG constitutes 75% of serum immunoglobulins in humans. IgG molecules are synthesized and secreted by plasma B cells. |
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