Dopamine beta Hydroxylase antibody (70R-12594)

Affinity purified Rabbit polyclonal Dopamine beta Hydroxylase antibody

Synonyms Polyclonal Dopamine beta Hydroxylase antibody, Anti-Dopamine beta Hydroxylase antibody, dopamine beta-hydroxylase antibody, DBM antibody, dopamine beta-hydroxylase (dopamine beta-monooxygenase) antibody, DBH antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB
Immunogen Dopamine beta Hydroxylase antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 8 and 320 of Dopamine beta Hydroxylase

Images

Immunofluorescent staining using Dopamine beta Hydroxylase antibody (70R-12594)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Dopamine beta-Hydroxylase antibody at 1:200 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification Dopamine beta Hydroxylase antibody was purified by antigen-affinity chromatography
Molecular Weight 69 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IHC-P: 1:100-1:500, WB: 1:1000-1:10000, IF:1:100-1:200, ICC:1:100-1:200

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using Dopamine beta Hydroxylase antibody (70R-12594)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Dopamine beta-Hydroxylase antibody at 1:200 dilution.
  • <div><font face=Calibri size=2>Western blot analysis using Dopamine beta Hydroxylase antibody (70R-12594)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: Hep G2) using a 7.5 % SDS PAGE gel and Dopamine beta Hydroxylase antibody at a dilution of 1:5000
  • <div><font face=Calibri size=2>Immunohistochemical staining using Dopamine beta Hydroxylase antibody (70R-12594)</font></div>     | Immunohistochemical staining of paraffin-embedded SW480 xenograft using Dopamine beta Hydroxylase antibody at a dilution of 1:500

Availability: In stock

Price: €323.11
Size: 100 ul
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