DPP4 protein (30R-1195)

Purified recombinant Human DPP4 protein

Synonyms DPP 4 protein, DPPIV protein, DPP4 protein, DPP4, DPP 4, CD26 protein, ADCP2 protein, dipeptidylpeptidase IV (CD26 adenosine deaminase complexing protein 2) protein, adenosine deaminase complexing protein 2 protein, , TP103 protein, DPP -4 protein, ADABP protein, Dipeptidyl peptidase-4 protein, DPP -4, dipeptidyl-peptidase 4 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare a 80ul reaction mix in a suitable container: The final concentrations are 1mM EDTA,20mM Tris pH8.0, 0.1M NaCl. 2. Add 10 ul of 10mM substrate (Gly-Pro p-nitroanilide) to each well. 3. Add 10ul of recombinant DPP-4 diluent to each well. 4. Incubate the 96 well plate at 37 DEG C for 10 min. 5. Read the optical density at 405 nm.

Images

Coomassie Blue stained SDS-PAGE of DPP4 protein (30R-1195)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 39-766 amino acids: ADP-R KTYTLTDYLK NTYRLKLYSL RWISDHEYLY KQENNILVFN AEYGNSSVFL ENSTFDEFGH SINDYSISPD GQFILLEYNY VKQWRHSYTA SYDIYDLNKR QLITEERIPN NTQWVTWSPV GHKLAYVWNN DIYVKIEPNL PSYRITWTGK EDIIYNGITD WVYEEEVFSA YSALWWSPNG TFLAYAQFND TEVPLIEYSF YSDESLQYPK TVRVPYPKAG AVNPTVKFFV VNTDSLSSVT NATSIQITAP ASMLIGDHYL CDVTWATQER ISLQWLRRIQ NYSVMDICDY DESSGRWNCL VARQHIEMST TGWVGRFRPS EPHFTLDGNS FYKIISNEEG YRHICYFQID KKDCTFITKG TWEVIGIEAL TSDYLYYISN EYKGMPGGRN LYKIQLSDYT KVTCLSCELN PERCQYYSVS FSKEAKYYQL RCSGPGLPLY TLHSSVNDKG LRVLEDNSAL DKMLQNVQMP SKKLDFIILN ETKFWYQMIL PPHFDKSKKY PLLLDVYAGP CSQKADTVFR LNWATYLAST ENIIVASFDG RGSGYQGDKI MHAINRRLGT FEVEDQIEAA RQFSKMGFVD NKRIAIWGWS YGGYVTSMVL GSGSGVFKCG IAVAPVSRWE YYDSVYTERY MGLPTPEDNL DHYRNSTVMS RAENFKQVEY LLIHGTADDN VHFQQSAQIS KALVDVGVDF QAMWYTDEDH GIASSTAHQH IYTHMSHFIK QCFSLP-SGRLVPRGSHHHHHH
Expression System Hi-5 cells
Grade & Purity > 95% pure
Molecular Weight 86.4 kDa (746aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in 20mM Tris-HCl, pH 8.0, containing 100mM NaCl, 1mM EDTA, and 10% glycerol
Concentration 0.5 mg/ml

Usage & Assay Information

Bioactivity Approximately > 50 Unit/mg. One unit will hydrolyze 1 uM of p-nitroaniline per minute at pH 8.0 and 37 DEG C using 1mM of Gly-Pro p-nitroanilde as a substrate.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method)

General Information

Biological Significance DPP4, dipeptidyl peptidase-4 is a complex enzyme expressed on the surface of most cell types and is a serine exopeptidase that cleaves x-proline dipeptides from the N-terminus of polypeptides. DPP4 protein is associated with intracellular signal transduction, apoptosis and plays an important role in tumor biology. There are at least 63 substrates which can bind specifically to DPP4 enzyme including growth factors, chemokines, neuro peptides. Furthermore, DPP4 plays a major role in glucose metabolism by cleaving incretins such as glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1). Recombinant human DPP4 protein was expressed with c-terminal His-tag in high-5 cells using baculovirus expression system and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of DPP4 protein (30R-1195) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €371.64
Size: 100 ug
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