DUT protein (His tag) (80R-1487)

Purified recombinant Human DUT protein

Synonyms dUTP pyrophosphatase protein, dUTPase protein, dUTP pyrophosphatase protein, FLJ20622 protein, dUTPase protein, FLJ20622
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of DUT protein (His tag) (80R-1487)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 70-252 amino acids: MGSSHHHHHH SSGLVPRGSH MASTVGAAGW KGELPKAGGS PAPGPETPAI SPSKRARPAE VGGMQLRFAR LSEHATAPTR GSARAAGYDL YSAYDYTIPP MEKAVVKTDI QIALPSGCYG RVAPRSGLAA KHFIDVGAGV IDEDYRGNVG VVLFNFGKEK FEVKKGDRIA QLICERIFYP EIEEVQALDD TERGSGGFGS TGKN
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 21.6 kDa (204aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 1mM DTT and 0.1M NaCl.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance DUT, also known as dUTP pyrophosphatase, is a ubiquitous enzyme that functions in nucleotide metabolism. This protein, in the presence of magnesium ions, is responsible for hydrolyzing dUTP to dUMP and diphosphate. This reaction is important for keeping the intracellular dUTP concentration low so that uracil does not become incorporated into DNA. Extensive incorporation of uracil into DNA can ultimately lead to cell death. This suggests that DUT is essential for cell viability, further implying that it is a potential target for anticancer therapy. Recombinant human DUT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of DUT protein (His tag) (80R-1487) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €239.92
Size: 100 ug
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