E Cadherin antibody (70R-49518)

Purified Polyclonal E Cadherin antibody

Synonyms Polyclonal E Cadherin antibody, Anti-E Cadherin antibody, CDH1 antibody, CDHE antibody, UVO antibody, Cadherin-1 antibody, CAM 120/80 antibody, Epithelial cadherin antibody, E-cadherin antibody, Uvomorulin antibody, CD324 antibody, CDH2 antibody, CDHN antibody, NCAD antibody, Cadherin-2 antibody, CDw325 antibody, Neural cadherin antibody, N-cadherin antibody, CD325 antibody, CDH3 antibody, CDHP antibody, Cadherin-3 antibody, Placental cadherin antibody, P-cadherin antibody, CDH4 antibody, Cadherin-4 antibody, Retinal cadherin antibody, R-CAD antibody, R-cadherin antibody
Specificity Recognizes endogenous levels of E Cadherin protein
Cross Reactivity Rat, Bovine, Dog, Monkey, Pig, Rabbit
Applications FC, ICC, IF, IHC, IP, WB
Immunogen E Cadherin antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human E Cadherin as the immunogen

Images

Western blot analysis using E Cadherin antibody (70R-49518)

Western blot analysis of E Cadherin expression in HEK293T (A); PC12 (B); A431 (C); MCF7 (D); C2C12 (E) whole cell lysates.

Specifications

Host Rabbit
Method of Purification E Cadherin antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IF: 1:100 - 1:500, ICC: 1:100 - 1:500, IP: 1:10 - 1:100, FC: 1:100 - 1:200

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance E Cadherin is expressed in most normal epithelial tissues. Selective loss of E-cadherin can cause dedifferentiation and invasiveness in human carcinomas, leading E-cadherin to be classified as a tumor suppressor.

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Images

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Western blot analysis using E Cadherin antibody (70R-49518)



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Western blot analysis of E Cadherin expression in HEK293T (A); PC12 (B); A431 (C); MCF7 (D); C2C12 (E) whole cell lysates.
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Immunoprecipitation analysis using E Cadherin antibody (70R-49518)



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Immunoprecipitation of E Cadherin from 0.5mg HEK293F whole cell extract lysate using 5ug of Anti-E Cadherin Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min HEK293F whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70 C; 10ul of each sample was separated on a SDS PAGE gel transferred to a nitrocellulose membrane blocked with 5% BSA and probed with Anti-E Cadherin Antibody.
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Immunohistochemical analysis using E Cadherin antibody (70R-49518)



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Immunohistochemical analysis of E Cadherin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis using E Cadherin antibody (70R-49518)



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Immunofluorescent analysis of E Cadherin staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Price: €197.96
Size: 100 ul
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