Ebola Virus NP protein (80-1411)

Purified Recombinant Ebola Nucleoprotein antigen (Zaire strain)

Synonyms Ebola Virus Nucleoprotein, Ebola protein, Ebola virus antigen, Zaire Ebola nucleoprotein antigen, Zaire NP protein, Ebola Zaire nucleoprotein antigen, Ebola virus protein
Species Viral
Protein Type Recombinant
Applications SDS-PAGE, WB
Assay Information Ebola Virus antibody (10-2254) can be used to detect this antigen

Images

SDS Page analysis using Ebola Virus NP protein (80-1411)

Lane annotations donate how much protein was loaded. Left lane: 250 ng protein; Right Lane: 500 ng protein

Specifications

Residues 1-739 aa (GenBank: AHX24646.1)
Expression System E.coli
Grade & Purity > 90% pure
Method of Purification Purified using proprietary chromatographic techniques
Molecular Weight 87 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in 50mM Tris buffer,pH 8.5, 200mM NaCl, 8M Urea, 2mM DTT and1mM EDTA

Storage & Safety

Storage Store at 4 deg C for shor term storage, for long term storage, aliquot and store at -70 deg C. Avoid repeated freeze/thaw cycles.

General Information

Product Use Purified Recombinant Ebola Virus Nucleoprotein for use in SDS-PAGE and Western Blot.
Biological Significance The Ebola Virus (EBOV) causes severe hemorrhagic fever with a mortality rate of up to 90%. EBOV is a member of the order Mononegavirales and, like other viruses in this taxonomic group, contains a negative-sense single-stranded (ss) RNA. The EBOV ssRNA encodes seven distinct proteins. One of them, the nucleoprotein (NP), is the most abundant viral protein in the infected cell and within the viral nucleocapsid. Like other EBOV proteins, NP is multifunctional. It is tightly associated with the viral genome and is essential for viral transcription, RNA replication, genome packaging and nucleocapsid assembly prior to membrane encapsulation.
Expression The viral RNA dependent RNA polymerase binds the encapsidated genome at the leader region, then sequentially transcribes each genes by recognizing start and stop signals flanking viral genes. mRNAs are capped and polyadenylated by the L protein during synthesis. The primary product of the unedited transcript of GP gene yields a smaller non-structural glycoprotein sGP which is efficiently secreted from infected cells. RNA editing allows expression of full-length GP.
Implications in Disease Typically, Ebola virus infection runs its course within 14 to 21 days. Infection initially presents with nonspecific flu-like symptoms such as fever, myalgia, and malaise. As the infection progresses, patients exhibit severe bleeding and coagulation abnormalities, including gastrointestinal bleeding, rash, and a range of hematological irregularities, such as lymphopenia and neutrophilia. Cytokines are released when reticuloendothelial cells encounter virus, which can contribute to exaggerated inflammatory responses that are not protective. Damage to the liver, combined with massive viremia, leads to disseminated intravascular coagulopathy. The virus eventually infects microvascular endothelial cells and compromises vascular integrity. The terminal stages of Ebola virus infection usually include diffuse bleeding, and hypotensive shock accounts for many Ebola virus fatalities.
Sequence Information The Ebola nucleoprotein is a homooligomer found on the Ebola virus. It homomultimerizes to form the nucleocapsid. At 739 amino acids, the nucleoprotein (NP) of Ebola virus is the largest nucleoprotein of the nonsegmented negative-stranded RNA viruses, and like the NPs of other viruses, it plays a central role in virus replication. The predicted coding region is 2217 bases in length and encodes a protein that contains 739 amino acids, with a calculated molecular weight of 83.3 kDa. The protein has an approximate net charge of -30 and can be divided into a hydrophobic N-terminal half and a hydrophilic and highly acidic C-terminal half.
Post-Translational Modifications Ebola nucleoprotein is Phosphorylated and O-glycosylated by the host organisms cells.
Database Links http://www.ncbi.nlm.nih.gov/protein/AHX24646.1

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Images

  • SDS Page analysis using Ebola Virus NP protein (80-1411) | Lane annotations donate how much protein was loaded. Left lane: 250 ng protein; Right Lane: 500 ng protein
  • Western Blot analysis using Ebola Virus NP protein (80-1411) | 250 ng of Ebola NP protein was loaded onto the gel and transferred to nitrocellulose membrane which was probed with an anti-his antibody at a dilution of 1:1000
  • Western Blot analysis using Ebola Virus antibody (10-2254) to detect Ebola Virus NP protein (80-1411) | 500 ng of Ebola NP protein was loaded onto the gel and transferred to nitrocellulose membrane which was probed with an Ebola Virus antibody (10-2254) at a dilution of 1:1000.

Availability: In stock

Price: €110.43
Size: 100 ug
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