Ebola Virus VP40 protein (30-1837)

Recombinant Ebola Virus VP40 Matrix protein

Synonyms Recombinant Ebola Virus VP40 protein, Ebola Virus VP40 Matrix protein, Ebola VP40 protein, Ebola Zaire protein
Species Viral
Protein Type Recombinant
Applications ELISA, Lateral Flow, SDS-PAGE, WB
Assay Information This Ebola VP40 matrix protein is highly reactive with the VP40 antibody pair, 10-2351 and 10-2352 and all three components are excellent reagents for optimizing immunoassays for Ebola virus.

Images

Western Blot analysis using Ebola Virus VP40 protein (30-1837)

100 ng of Ebola Virus VP40 protein (30-1837) was loaded, antibody dilution was 1:3000

Specifications

Residues 1-326 aa of Ebola VP40 protein with N-terminal Hexahistidine and GST tags. Zaire strain, Accession No. AHX24648
Expression System E.coli
Grade & Purity > 90% pure
Method of Purification Purified using proprietary chromatographic techniques
Molecular Weight 68 kDa
Tag/Conjugate His-GST tag
Form & Buffer Suppliedas liquid in 50mM Tris pH 8.5, 150mM NaCl with 10% glycerol, 2mM DTT and 1mM EDTA

Usage & Assay Information

Usage Recommendations To be optimized by the end user

Storage & Safety

Storage Ships on dry ice. Upon receipt, aliquot and store at -70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The Ebola VP40 protein is a viral matrix protein. VP40 coordinates numerous functions in the viral life cycle of the Ebola virus such as,regulation of viral transcription, morphogenesis,andpackaging and budding of mature virions. The VP40 monomer consists of two protein domains, the N-terminal oligomerization domain and the C-terminal membrane-binding domain, connected by a flexible linker. Study of the matrix protein VP40 is important due to the high mortality rate of the Ebola virus, which is listed as a WHO Risk Group 4 Pathogen, an HHS Select Agent, an NIH/NIAID Category A Priority Pathogen, a CDC Category A Bioterrorism Agent, and a Biological Agent for Export Control by the Australia Group
Database Links http://www.ncbi.nlm.nih.gov/protein/AHX24648

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Images

  • Western Blot analysis using Ebola Virus VP40 protein (30-1837) | 100 ng of Ebola Virus VP40 protein (30-1837) was loaded, antibody dilution was 1:3000
  • SDS-PAGE analysis of Ebola VP40 protein (30-1837) | Left lane: 250 ng VP40 protein; Middle lane: Molecular weight marker; Right lane: 500 ng VP40 protein. Expected molecular weight of 68 kDa.
  • Western blot analysis of Ebola VP40 protein (30-1837) | 250 ng (left lane) and 500 ng (right lane) were resolved on an SDS-PAGE and transferred to nitrocellulose membrane. The blot was probed with an anti-his antibody. A strong band of approximately 68 kDa was observed, along with a weaker band of approximately 25 kDa which represents an insignificantamount of degradation product.
  • SDS-PAGE analysis of Ebola VP40 protein (30-1837) | Left lane: 250 ng VP40 protein; Middle lane: Molecular weight marker; Right lane: 500 ng VP40 protein. Expected molecular weight of 68 kDa.
  • Western blot analysis of Ebola VP40 protein (30-1837) | 250 ng (left lane) and 500 ng (right lane) were resolved on an SDS-PAGE and transferred to nitrocellulose membrane. The blot was probed with an anti-his antibody. A strong band of approximately 68 kDa was observed, along with a weaker band of approximately 25 kDa which represents an insignificant amount of degradation product.

Availability: In stock

Price: €110.43
Size: 100 ug
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