EBV EBNA1 protein (30R-AE006)

Purified recombinant EBV EBNA1 protein

Synonyms Epstein Barr Nuclear Antigen 1 protein, EBV HHV4 EBNA1 protein, Epstein-Barr Virus EBNA 1 protein
Species Viral
Protein Type Recombinant
Applications ELISA, WB

Specifications

Residues The E.Coli derived recombinant mosaic protein contains the HHV-4 EBNA regions, 1-90, 408-498 amino acids, the Mw is 46kDa (including 26kDa GST tag).
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 46 kDa (including GST tag of 26 kDa)
Tag/Conjugate GST tag
Form & Buffer 50mM tris-HCl, pH 8.0, with 10mM glutation, 60mM NaCl and 0.5% sarcosyl.

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The Epstein-Barr virus (EBV), also called Human herpes virus 4 (HHV-4), is a virusof the herpes family (which includes Herpes simplex virusand Cytomegalo virus. On infecting the B-lymphocyte, the linear virus genome circularizes and the virus subsequently persists within the cell as an episome. The virus can execute several distinct programs of gene expressionwhich can be broadly categorized as being lytic cycle or latent cycle. The lytic cycleor productive infection results in staged expression of a host of viral proteinswith the ultimate objective of producing infectious virions. Formally, this phase of infection does not inevitably lead to lysis of the host cellas EBV virions are produced by budding from the infected cell. The latent cycle(lysogenic) programs are those that do not result in production of virions.

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