EBV p54 protein (His tag) (80-1361)

Purified recombinant EBV p54 protein (His tag)

Synonyms p54 early antigen, BMRF1 protein, UL42 protein, Epstein-Barr virus p54 protein, EBV p54 protein, EBV early antigen, Epstein-Barr virus p54
Species Viral
Protein Type Recombinant
Applications Dot Blot, ELISA, WB

Specifications

Residues p54 expressed as a recombinant antigen fused to a his-tag at the N-terminal. Produced from the fragment between 771 to 1212 pb of the EBV BMRF1 gene, corresponding to amino acids 257-404 of the C-terminal of the protein
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 20.342 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in 20 mM phosphate buffer, pH 8.0, with 1 M NaCl and 0.1% polyoxyethelene (10) tridecyl ether and 6 mM urea
Concentration ~ 0.5 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:500
Bioactivity Immunoreactive with sera of EBV IgM positive individuals

Storage & Safety

Storage Store at -20 deg C to -80 deg C, avoid repeated freeze/thaw cycles

General Information

Biological Significance The D-EA p47/54 is an antigen codified by the BMRF1 gene, which is a DNA polymerase processivity factor. At the time of infection with Epstein-Barr virus, antibodies to EA are found and usually last for four to six months only. The absence of antibody to EA when other EBV antibodies are present strongly suggests that first time infection with EBV occurred in the past.

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