EBV p54 protein (His tag) (80-1361)
Purified recombinant EBV p54 protein (His tag)
Overview
Overview
| Synonyms | p54 early antigen, BMRF1 protein, UL42 protein, Epstein-Barr virus p54 protein, EBV p54 protein, EBV early antigen, Epstein-Barr virus p54 |
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| Species | Viral |
| Protein Type | Recombinant |
| Applications | Dot Blot, ELISA, WB |
Specifications
| Residues | p54 expressed as a recombinant antigen fused to a his-tag at the N-terminal. Produced from the fragment between 771 to 1212 pb of the EBV BMRF1 gene, corresponding to amino acids 257-404 of the C-terminal of the protein |
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| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Molecular Weight | 20.342 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied in 20 mM phosphate buffer, pH 8.0, with 1 M NaCl and 0.1% polyoxyethelene (10) tridecyl ether and 6 mM urea |
| Concentration | ~ 0.5 mg/ml |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:500 |
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| Bioactivity | Immunoreactive with sera of EBV IgM positive individuals |
Storage & Safety
| Storage | Store at -20 deg C to -80 deg C, avoid repeated freeze/thaw cycles |
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General Information
| Biological Significance | The D-EA p47/54 is an antigen codified by the BMRF1 gene, which is a DNA polymerase processivity factor. At the time of infection with Epstein-Barr virus, antibodies to EA are found and usually last for four to six months only. The absence of antibody to EA when other EBV antibodies are present strongly suggests that first time infection with EBV occurred in the past. |
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