Ecotin protein (His tag) (80R-1637)
Purified recombinant E.coli Ecotin protein
Overview
Overview
| Synonyms | JW2197, ECK2201 protein, eti protein |
|---|---|
| Species | E.coli |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of Ecotin protein (His tag) (80R-1637)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 21-162 amino acids: MGSSHHHHHH SSGLVPRGSH MAESVQPLEK IAPYPQAEKG MKRQVIQLTP QEDESTLKVE LLIGQTLEVD CNLHRLGGKL ENKTLEGWGY DYYVFDKVSS PVSTMMACPD GKKEKKFVTA YLGDAGMLRY NSKLPIVVYT PDNVDVKYRV WKAEEKIDNA VVR |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Molecular Weight | 18.3 kDa (163aa), confirmed by MALDI-TOF |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 50mM NaCl, 1mM DTT and 10% glycerol. |
| Concentration | 1 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | Ecotin (E. coli serine protease inhibitor) inhibits pancreatic serine proteases. This protein inhibits chymotrypsin, trypsin, elastases, factor X, kallikrein as well as a variety of other proteases. The strength of inhibition does not appear to be correlated with a particular protease specificity. Recombinant E. coli Ecotin protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
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