EDF1 protein (His tag) (80R-1417)

Purified recombinant Human EDF1 protein

Synonyms Endothelial differentiation-related factor 1, EDF-1, EDF 1 protein, EDF1, EDF-1 protein, EDF-1 protein, EDF 1, MBF1 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of EDF1 protein (His tag) (80R-1417)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-148 amino acids: MAESDWDTVT VLRKKGPTAA QAKSKQAILA AQRRGEDVET SKKWAAGQNK QHSITKNTAK LDRETEELHH DRVTLEVGKV IQQGRQSKGL TQKDLATKIN EKPQVIADYE SGRAIPNNQV LGKIERAIGL KLRGKDIGKP IEKGPRAKLE HHHHHH
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 17.4 kDa (156aa) confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 20% glycerol and 0.1M NaCl.
Concentration 0.5 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Endothelial differentiation-related factor 1, also known as EDF1 may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. Also, EDF1 binds calmodulin thorough its IQ domain and regulates nitric oxide synthase activity through calmodulin sequestration in the cytoplasm. Though ubiquitously expressed, EDF1 is most abundant in adult liver, heart, adipose tissues, intestine and pancreas. Recombinant human EDF1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of EDF1 protein (His tag) (80R-1417) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €239.92
Size: 100 ug
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