EED antibody (70R-13478)

Affinity purified Rabbit polyclonal EED antibody

Synonyms Polyclonal EED antibody, Anti-EED antibody, polycomb protein EED antibody, WD protein associating with integrin cytoplasmic tails 1 antibody, embryonic ectoderm development antibody, WAIT1 antibody, HEED antibody, EED antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB

Images

Immunofluorescent staining using EED antibody (70R-13478)

Immunofluorescence analysis of paraformaldehyde-fixed A431, using EED antibody at 1:500 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification EED antibody was purified by antigen-affinity chromatography
Molecular Weight 50 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.47 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance This gene encodes a member of the Polycomb-group family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using EED antibody (70R-13478)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed A431, using EED antibody at 1:500 dilution.
  • <div><font face=Calibri size=2>Western blot analysis using EED antibody (70R-13478)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: A431; B: H1299) using a 10 % SDS PAGE gel and EED antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunohistochemical staining using EED antibody (70R-13478)</font></div>     | Immunohistochemical staining of paraffin-embedded SAS Xenograft using EED antibody at a dilution of 1:100

Availability: In stock

Price: €323.11
Size: 100 ul
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