EIF1 protein (His tag) (80R-1244)
Purified recombinant Human EIF1 protein
|Synonyms||EIF1A protein, EIF-1, EIF 1, ISO1 protein, SUI1 protein, EIF1, EIF1 protein, , EIF 1 protein, EIF-1 protein, EIF-1 protein, Eukaryotic translation initiation factor 1 protein, A121 protein|
Coomassie Blue stained SDS-PAGE of EIF1 protein (His tag) (80R-1244)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-113 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMSA IQNLHSFDPF ADASKGDDLL PAGTEDYIHI RIQQRNGRKT LTTVQGIADD YDKKKLVKAF KKKFACNGTV IEHPEYGEVI QLQGDQRKNI CQFLVEIGLA KDDQLKVHGF|
|Grade & Purity||> 90% pure|
|Molecular Weight||16.9 kDa (150aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 0.1 M NaCl and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||EIF1, also known as eukaryotic translation initiation factor 1, is a universally conserved translation factor that is necessary for scanning and involved in initiation site selection. This protein promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA. Recombinant human EIF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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