EIF3F antibody (70R-50623)
Purified Polyclonal EIF3F antibody
Overview
Overview
| Synonyms | Polyclonal EIF3F antibody, Anti-EIF3F antibody, EIF3S5 antibody, Eukaryotic translation initiation factor 3 subunit F antibody, eIF3f antibody, Deubiquitinating enzyme eIF3f antibody, Eukaryotic translation initiation factor 3 subunit 5 antibody, eIF-3-epsilon antibody, eIF3 p47 antibody |
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| Specificity | Recognizes endogenous levels of EIF3F protein |
| Cross Reactivity | Mouse, Rat, Monkey |
| Applications | IP, WB |
| Immunogen | EIF3F antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EIF3F as the immunogen |
Images
Western blot analysis using EIF3F antibody (70R-50623)
Western blot analysis of EIF3F expression in HeLa (A); HepG2 (B); NIH3T3 (C) whole cell lysates.
Specifications
| Host | Rabbit |
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| Method of Purification | EIF3F antibody was purified by immunogen affinity chromatography |
| Form & Buffer | Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide |
Usage & Assay Information
| Usage Recommendations | WB: 1:500 - 1:1000, IP: 1:10 - 1:100 |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles |
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General Information
| Biological Significance | EIF3F is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). |
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