EIF3K protein (His tag) (80R-1730)
Purified recombinant Human EIF3K protein
|Synonyms||Eukaryotic translation initiation factor 3 subunit K protein, PTD001 protein, ARG134 protein, HSPC029 protein, M9 protein, PLAC24 protein, PRO1474 protein, EIF3-p28 protein, MSTP001 protein, EIF3K, EIF3S12 protein, EIFK 3 protein, PLAC-24 protein, EIFK-3, EIFK-3 protein, EIFK 3|
Coomassie Blue stained SDS-PAGE of EIF3K protein (His tag) (80R-1730)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-218 amino acids: MGSSHHHHHH SSGLVPRGSH MAMFEQMRAN VGKLLKGIDR YNPENLATLE RYVETQAKEN AYDLEANLAV LKLYQFNPAF FQTTVTAQIL LKALTNLPHT DFTLCKCMID QAHQEERPIR QILYLGDLLE TCHFQAFWQA LDENMDLLEG ITGFEDSVRK FICHVVGITY QHIDRWLLAE MLGDLSDSQL KVWMSKYGWS ADESGQIFIC SQEESIKPKN IVEKIDFDSV SSIMASSQ|
|Grade & Purity||> 95% pure|
|Molecular Weight||27.2 kDa (238aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 0.1M NaCl and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||EIF3K, also known as eukaryotic translation initiation factor 3 subunit K, belongs to the eIF3 subunit K family. It is the smallest subunit of eIF3 and it interacts with several other subunits of eIF3 and the 40S ribosomal subunit. This protein is conserved among high eukaryotes, including mammals, insects, and plants, and it is ubiquitously expressed in human tissues. It is distributed both in nucleus and cytoplasm and colocalizes with cyclin D3, a regulatory subunit of cyclin-dependent kinase 4 (Cdk4). Recombinant human EIF3K protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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