Ephrin A3 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Ephrin A3 as the immunogen
Western blot analysis using Ephrin A3 antibody (70R-49686)
Western blot analysis of Ephrin A3 expression in HeLa (A); HepG2 (B) whole cell lysates.
Ephrin A3 antibody
Western blot analysis using Ephrin A3 antibody (70R-49686)
Western blot analysis of Ephrin A3 expression in HeLa (A); HepG2 (B) whole cell lysates.
Immunofluorescent analysis using Ephrin A3 antibody (70R-49686)
Immunofluorescent analysis of Ephrin A3 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Specifications
Host
Rabbit
Method of Purification
Ephrin A3 antibody was purified by immunogen affinity chromatography
Form & Buffer
Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide
Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles
General Information
Biological Significance
Ephrin A3 is a cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development.