Ephrin A3 antibody (70R-49686)

Purified Polyclonal Ephrin A3 antibody

Synonyms Polyclonal Ephrin A3 antibody, Anti-Ephrin A3 antibody, EFL2 antibody, EPLG3 antibody, LERK3 antibody, Ephrin-A3 antibody, EFL-2 antibody, EHK1 ligand antibody, EHK1-L antibody, EPH-related receptor tyrosine kinase ligand 3 antibody, LERK-3 antibody
Specificity Recognizes endogenous levels of Ephrin A3 protein
Cross Reactivity Mouse, Rat
Applications FC, ICC, IF, WB
Immunogen Ephrin A3 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Ephrin A3 as the immunogen

Images

Western blot analysis using Ephrin A3 antibody (70R-49686)

Western blot analysis of Ephrin A3 expression in HeLa (A); HepG2 (B) whole cell lysates.

Specifications

Host Rabbit
Method of Purification Ephrin A3 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IF: 1:100 - 1:500, ICC: 1:100 - 1:500, FC: 1:100 - 1:200

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance Ephrin A3 is a cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development.

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Images

  • 

Western blot analysis using Ephrin A3 antibody (70R-49686)



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Western blot analysis of Ephrin A3 expression in HeLa (A); HepG2 (B) whole cell lysates.
  • 

Immunofluorescent analysis using Ephrin A3 antibody (70R-49686)



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Immunofluorescent analysis of Ephrin A3 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Availability: In stock

Price: €197.96
Size: 100 ul
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